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首页> 外文OA文献 >Microtubule-Associated Protein AtMPB2C Plays a Role in Organization of Cortical Microtubules, Stomata Patterning, and Tobamovirus Infectivity1
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Microtubule-Associated Protein AtMPB2C Plays a Role in Organization of Cortical Microtubules, Stomata Patterning, and Tobamovirus Infectivity1

机译：微管相关蛋白AtMPB2C在皮层微管，气孔模式和烟草花叶病毒的感染性1的组织中发挥作用。

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AtMPB2C is the Arabidopsis (Arabidopsis thaliana) homolog of MPB2C, a microtubule-associated host factor of tobacco mosaic virus movement protein that was been previously identified in Nicotiana tabacum. To analyze the endogenous function of AtMPB2C and its role in viral infections, transgenic Arabidopsis plant lines stably overexpressing green fluorescent protein (GFP)-AtMPB2C were established. The GFP-AtMPB2C fusion protein was detectable in various cell types and organs and localized at microtubules in a punctuate pattern or in filaments. To determine whether overexpression impacted on the cortical microtubular cytoskeleton, GFP-AtMPB2C-overexpressing plants were compared to known microtubular marker lines. In rapidly elongated cell types such as vein cells and root cells, GFP-AtMPB2C overexpression caused highly unordered assemblies of cortical microtubules, a disturbed, snake-like microtubular shape, and star-like crossing points of microtubules. Phenotypically, GFP-AtMPB2C transgenic plants showed retarded growth but were viable and fertile. Seedlings of GFP-AtMPB2C transgenic plants were characterized by clockwise twisted leaves, clustered stomata, and enhanced drought tolerance. GFP-AtMPB2C-overexpressing plants showed increased resistance against oilseed rape mosaic virus, a close relative of tobacco mosaic virus, but not against cucumber mosaic virus when compared to Arabidopsis wild-type plants. These results suggest that AtMPB2C is involved in the alignment of cortical microtubules, the patterning of stomata, and restricting tobamoviral infections.
机译：AtMPB2C是MPB2C的拟南芥（Arabidopsis thaliana）同源物，MPB2C是烟草花叶病毒运动蛋白的微管相关宿主因子，先前已在烟草中鉴定出。为了分析AtMPB2C的内源功能及其在病毒感染中的作用，建立了稳定过量表达绿色荧光蛋白（GFP）-AtMPB2C的转基因拟南芥植物品系。 GFP-AtMPB2C融合蛋白可在各种细胞类型和器官中检测到，并以点状或细丝形式位于微管中。为了确定过表达是否影响皮质微管细胞骨架，将GFP-AtMPB2C-过表达植物与已知的微管标记物系进行了比较。在快速伸长的细胞类型（如静脉细胞和根细胞）中，GFP-AtMPB2C的过表达导致皮质微管的高度无序排列，紊乱的蛇形微管形状和微管的星形交叉点。从表型上看，GFP-AtMPB2C转基因植物显示出生长受阻，但具有活力和繁殖力。 GFP-AtMPB2C转基因植物的幼苗具有顺时针扭曲的叶片，成簇的气孔和增强的耐旱性的特征。与拟南芥野生型植物相比，过表达GFP-AtMPB2C的植物显示出对油菜籽花叶病毒（烟草花叶病毒的近亲）的抗性增强，但对黄瓜花叶病毒没有抗性。这些结果表明AtMPB2C参与皮层微管的排列，气孔的形成以及限制烟草花叶病毒的感染。

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